The Group for Muscle Cellular and Molecular Biology investigates different aspects of the structure and function of striated muscle - heart and skeletal muscle. Of our particular interest are molecular mechanisms of:
Central role in studying the processes involved in recovery of muscle tissue after injury and adaptive response to stress belongs to the genes encoding members of the family of muscle proteins with ankyrin repeats (Muscle Ankyrin Repeat Proteins, MARP). ANKRD1 and ANKRD2 participate in mechanotransduction of signals from the sarcomere to the nucleus, where they modulate the expression of target genes. They are expressed during the development of the heart and skeletal muscle, upon increased mechanical load and in pathological conditions, such as myocardial infarction and myopathies. By profiling their expression, identification of interacting partners and signaling pathways in which they are involved, and deciphering their function we are getting deeper insight into mechanisms of muscle regeneration and repair, both in physiological and stress conditions. We analyze ANKRD1 and ANKRD2 genes as markers for the differential diagnosis of rhabdomyosarcoma, and consider them as potential therapeutic targets.
We employ the zebrafish (Danio rerio) as an in vivo model system. In order to decipher the function of genes under the study, we use mutants generated by Crispr / Cas9 technology and reporter lines. We established a model of myocardial infarction, mechanical injury of skeletal muscle, and model of increased physical activity by forced swimming. In addition to zebrafish, we use cell cultures and cadaveric human muscle samples. A wide range of methods and techniques for protein analysis, including western blot, immunohistochemistry, immunocytochemistry, methods for detection of protein-protein interactions (such as pull down and co-immunoprecipitation), as well as fluorescence and confocal microscopy are proficiently used in our research.
Vojvode Stepe 444a
P.O BOX 23
11010 Beograd, Srbija
Tel: +381 11 3976658
Mob: +381 66 8038500
Faks: +381 11 3975808