Cytotoxicity of natural or chemically synthesized compounds is determined using MTT assay on cells in culture. MTT assay is a colorimetric assay that measures the reduction of yellow 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase, which produces insoluble purple product formazan.
1. Cytotoxicity addressed on normal cells such as lung fibroblast cell line
2. Cytotoxicity addressed on several types of tumor cell lines
Result is provided in the form of IC50 concentration (compound concentration inhibiting 50 % cellular viability).
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