Milan Kojić

Principal Research Fellow

Institute of molecular genetics and genetic engineering (IMGGE),
University of Belgrade

V. Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia
Mobile: +381 64  499 86 68
Phone: +381 11 397 59 60
Fax:     +381 11 397 58 08
E-mail: mkojic@imgge.bg.ac.rs

EDUCATION

1995 - PhD in Molecular Genetics and Genetic Engineering, Faculty of Biology, University of Belgrade
1992 - M.Sc. in Molecular Genetics and Genetic Engineering, Faculty of Biology, University of Belgrade
1987 - B.Sc. in Biology, Faculty of Natural Sciences, University of Belgrade

RESEARCH EXPERIENCE

2007 - Principal Research Fellow,Laboratory for Molecular Microbiology, Institute of molecular genetics and genetic engineering, University of Belgrade
2002 - 2007 - Research Associate, Laboratory for Molecular Genetics of Industrial Microorganisms,Institute of molecular genetics and genetic engineering
1995 - 2002 - Research Assistant, Laboratory for Molecular Genetics of Industrial Microorganisms,Institute of molecular genetics and genetic engineering
1988 - 1895 - Research Trainee, Laboratory for Molecular Genetics of Industrial Microorganisms,Institute of molecular genetics and genetic engineering

CAREER HISTORY

2010 - Head of Laboratory for Molecular Microbiology, Institute of molecular genetics and genetic engineering, University of Belgrade

RESEARCH INTEREST

  • Regulation of gene expression in bacteria: in pseudomonads and lactic acid bacteria.
  • Cloning and expression of the genes in homologous and heterologous host systems for probiotic properties of lactic acid bacteria (production of bacteriocins, the serine type proteinase, aggregation factors and operons for synthesis of EPS
  • The study of the interaction of the bacteriocin molecule and the membrane receptor.
  • The study of the molecular mechanisms of antibiotic resistance in bacteria.

SELECTED PUBLICATIONS

Ovchinnikov KV, Kristiansen PE, Uzelac G, Topisirovic L, Kojic M, Nissen-Meyer J, Nes IF Diep DB., Defining the structure and receptor binding domain of the leaderless bacteriocin LsbB.
J Biol Chem. 2014 Aug 22;289(34):23838-45. doi: 10.1074/jbc.M114.579698.
PMID: 24993828

Lukic J, Strahinic I, Milenkovic M, Nikolic M, Tolinacki M, Kojic M, Begovic J.

Aggregation factor as an inhibitor of bacterial binding to gut mucosa.

Microb Ecol. 2014 Oct;68(3):633-44. doi: 10.1007/s00248-014-0426-1. Epub 2014 May 14.
PMID: 24823989
 

Uzelac G, Kojic M, Lozo J, Aleksandrzak-Piekarczyk T, Gabrielsen C, Kristensen T, Nes IF, Diep DB, Topisirovic L.

A Zn-dependent metallopeptidase is responsible for sensitivity to LsbB, a class II leaderless bacteriocin of Lactococcus lactis subsp. lactis BGMN1-5.

J Bacteriol. 2013 Dec;195(24):5614-21. doi: 10.1128/JB.00859-13. Epub 2013 Oct 11.
PMID: 24123824
 

Lukic J, Strahinic I, Milenkovic M, Golic N, Kojic M, Topisirovic L, Begovic J.

Interaction of Lactobacillus fermentum BGHI14 with rat colonic mucosa: implications for colitis induction.

Appl Environ Microbiol. 2013 Sep;79(18):5735-44. doi: 10.1128/AEM.01807-13. Epub 2013 Jul 12.
PMID: 23851097

Jovcic B, Lepsanovic Z, Begovic J, Rakonjac B, Perovanovic J, Topisirovic  L, Kojic M.

The clinical isolate Pseudomonas aeruginosa MMA83 carries two copies of the blaNDM-1 gene in a novel genetic context.

Antimicrob Agents Chemother. 2013 Jul;57(7):3405-7. doi: 10.1128/AAC.02312-12. Epub 2013 Apr 22.
PMID: 23612199
 

Lukic J, Strahinic I, Jovcic B, Filipic B, Topisirovic L, Kojic M, Begovic J.

Different roles for lactococcal aggregation factor and mucin binding protein in adhesion to gastrointestinal mucosa.

Appl Environ Microbiol. 2012 Nov;78(22):7993-8000. doi: 10.1128/AEM.02141-12. Epub 2012 Sep 7.
PMID: 22961901 
 

Nikolic M, Lopez P, Strahinic I, Suarez A, Kojic M, Ferna¡ndez-Garcia M, Topisirovic L, Golic N, Ruas-Madiedo P.

Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics.

Int J Food Microbiol. 2012 Aug 17;158(2):155-62. doi: 10.1016/j.ijfoodmicro.2012.07.015. Epub 2012 Jul 20.
PMID: 22858135
 

Kojic M, Jovcic B, Strahinic I, Begovic J, Lozo J, Veljovic K, Topisirovic L.

Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp. lactis BGKP1.

BMC Microbiol. 2011 Dec 19;11:265. doi: 10.1186/1471-2180-11-265.
PMID: 22182285

Jovcic B, Lepsanovic Z, Suljagic V, Rackov G, Begovic J, Topisirovic L, Kojic M.

Emergence of NDM-1 metallo-β-lactamase in Pseudomonas aeruginosa clinical isolates from Serbia.

Antimicrob Agents Chemother. 2011 Aug;55(8):3929-31. doi: 10.1128/AAC.00226-11. Epub 2011 Jun 6.
PMID: 21646490 

Begovic J, Huys G, Mayo B, D'Haene K, Florez AB, Lozo J, Kojic M, Strahinic I, Topisirovic L.

Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance.

Res Microbiol. 2009 Jul-Aug;160(6):421-6. doi: 10.1016/j.resmic.2009.07.005. Epub 2009 Jul 18.
PMID: 19619642
 

Lozo J, Jovcic B, Kojic M, Dalgalarrondo M, Chobert JM, Haertle T, Topisirovic L.

Molecular characterization of a novel bacteriocin and an unusually large aggregation factor of Lactobacillus paracasei subsp. paracasei BGSJ2-8, a natural isolate from homemade cheese.

Curr Microbiol. 2007 Sep;55(3):266-71. Epub 2007 Jul 25.
PMID: 17657532
 

Topisirovic L, Kojic M, Fira D, Golic N, Strahinic I, Lozo J.

Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation.

Int J Food Microbiol. 2006 Dec 1;112(3):230-5. Epub 2006 Jun 9.
PMID: 16764959
 

Kojic M, Jovcic B, Vindigni A, Odreman F, Venturi V.

Novel target genes of PsrA transcriptional regulator of Pseudomonas aeruginosa.

FEMS Microbiol Lett. 2005 May 15;246(2):175-81.
PMID: 15899403
 

Aguilar C, Friscina A, Devescovi G, Kojic M, Venturi V.

Identification of quorum-sensing-regulated genes of Burkholderia cepacia.

J Bacteriol. 2003 Nov;185(21):6456-62.
PMID: 14563881 
 

Pastar I, Tonic I, Golic N, Kojic M, van Kranenburg R, Kleerebezem M, Topisirovic L, Jovanovic G.

Identification and genetic characterization of a novel proteinase, PrtR, from the human isolate Lactobacillus rhamnosus BGT10.

Appl Environ Microbiol. 2003 Oct;69(10):5802-11.
PMID: 14532028 
 

Gajic O, Buist G, Kojic M, Topisirovic L, Kuipers OP, Kok J.

Novel mechanism of bacteriocin secretion and immunity carried out by lactococcal multidrug resistance proteins.

J Biol Chem. 2003 Sep 5;278(36):34291-8. Epub 2003 Jun 11.
PMID: 12801935
 

Kojic M, Aguilar C, Venturi V.

TetR family member psrA directly binds the Pseudomonas rpoS and psrA promoters.

J Bacteriol. 2002 Apr;184(8):2324-30.
PMID: 11914368 
 

Kojic M, Venturi V.

Regulation of rpoS gene expression in Pseudomonas: involvement of a TetR family regulator.

J Bacteriol. 2001 Jun;183(12):3712-20.
PMID: 11371535 [

Degrassi G, Kojic M, Ljubijankic G, Venturi V.

The acetyl xylan esterase of Bacillus pumilus belongs to a family of esterases with broad substrate specificity.

Microbiology. 2000 Jul;146 ( Pt 7):1585-91.
PMID: 10878123

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