Academician, Principal Research Fellow

Institute of molecular genetics and genetic engineering (IMGGE),
University of Belgrade

V. Stepe 444a, P.Fah 23, 11010 Belgrade, Serbia
Mobile:   +381 64  8044085                           
Phone:   +381 11 397 62 12
Fax:       +381 11 397 58 08
E-mail: milenastevanovic@imgge.bg.ac.rs

EDUCATION

1990 - PhD in Biological Sciences, Faculty of Biology (FB), UB (THESIS: Structural analysis of three haplotypes of β globin gene family encoding globine chains of hemoglobin in rats Rattus Norvegicus)

1987 - M.Sc. in Biological Sciences, FB, UB

1983 - B.Sc. in Molecular Biology, FB, University of Belgrade

RESEARCH EXPERIENCE

2000 - Principal Research Fellow Laboratory for Human Molecular Genetics (LHMG), IMGGE, UB

1996 - 2000 - Senior Research Associate LHMG, IMGGE, UB

1991 - 1996 - Research Associate LHMG, IMGGE, UB

1988 - 1991 - Research Assistant Genome Structure Unit (GSU), IMGGE

1986 - 1988 - Research Trainee GSU, IMGGE

CAREER HISTORY

2015 - Full member of Serbian Academy of Sciences and Arts

2009 - Member of Serbian Academy of Sciences and Arts

2007 - Lecturer on FB, UB, PhD studies in Molecular biology

2007 - Member of the Scientific board for Biology, Ministry of Education, Science and Technological Development, Republic of Serbia

2005 - 2014 - Member of the Steering Committee of the Serbian Biological Society

2004 - 2006 - Member of The University Council, UB

2003 - Member of the National Committee for Bioethics of UNESCO Commission of the Republic of Serbia

2001 - 2007 - Member of Committee for Biology, Ministry of Science and Technological Development, Republic of Serbia

2001 - 2006 - President of the Scientific Council, IMGGE

1999 - Lecturer on FB, UB, Bachelor Studies, course Basic gene manipulation

1999 - 2001 - Acting president of the Board, IMGGE

1994 - Head of Laboratory for Human Molecular Genetics (LHMG), IMGGE, UB

1993 - 2010 - Member of the Board, IMGGE

1992 - 1994 - Post-doc, Genetics Department, University of Cambridge, UK

1991 - 1992 - Post-doc, Imperial Cancer Research Fund, London, UK

1986 -1991 - Genome Structure Unit, IMGGE

RESEARCH INTEREST

Studying the structure and regulation of human SOX genes expression: identification of control elements involved in transcriptional regulation and analysis of the roles of Wnt and Sonic Hedgehog (SHH) signalling pathways in control of their expression; Studying the involvement of epigenetic mechanisms in the control of SOX genes expression; Investigation of the roles of SOX genes in maintaining pluripotency, cell fate determination and differentiation; Studying the roles of SOX genes in oncogenesis. High-resolution phylogenetic studies of mitochondrial DNA variation in contemporary Serbian population, as well as in ancient populations that lived in the Southwestern region of Serbia from Bronze Age up to Middle Ages; Detection of micro-deletion syndromes by Fluorescent in situ Hybridization (FISH).

SELECTED PUBLICATIONS

  1. Mojsin M., Topalovic V., Vicentic J.M., Schwirtlich M., Stanisavljevic D., Drakulic D., Stevanovic M. Crosstalk between SOXB1 proteins and WNT/β-catenin signaling in NT2/D1 cells. Histochem Cell Biol. 2015. 144:429-41. http://www.ncbi.nlm.nih.gov/pubmed/26239426
  1. Davidovic S., Malyarchuk B., Aleksic J.M., Derenko M., Topalovic V., Litvinov A., Stevanovic M., Kovacevic-Grujicic N. Mitochondrial DNA perspective of Serbian genetic diversity. Am J Phys Anthropol 2015. 156:449-465. http://www.ncbi.nlm.nih.gov/pubmed/25418795
  1. Petrovic, Milivojevic M., Popovic J., Schwirtlich M., Rankovic B., Stevanovic M. SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines.PLoS One. 2015. 10(11):e0143591. http://www.ncbi.nlm.nih.gov/pubmed/26588701
  1. Popovic J., Stanisavljevic D., Schwirtlich M., Klajn A., Marjanovic J., Stevanovic M. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. 2014. PLoS One, 9:e91852. http://www.ncbi.nlm.nih.gov/pubmed/24637840
  1. Mojsin M., Vicentic J.M., Schwirtlich M., Topalovic V., Stevanovic M. Quercetin reduces pluripotency, migration and adhesion of human teratocarcinoma cell line NT2/D1 by inhibiting Wnt/β-catenin signaling. 2014. Food Funct, 5:2564-73. http://www.ncbi.nlm.nih.gov/pubmed/25138740
  1. Popović V., Živković J., Davidović S., Stevanović M., Stojković D. Mycotherapy of cancer: an update on cytotoxic and antitumor activities of mushrooms, bioactive principles and molecular mechanisms of their action. 2013. Curr Top Med Chem,13:2791-806. http://www.ncbi.nlm.nih.gov/pubmed/24083788
  1. Drakulic D., Krstic A.,Stevanovic M. Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones. 2012. Genet Mol Res,11:1385-400. http://www.ncbi.nlm.nih.gov/pubmed/22653585
  1. CuturiloG., Menten B., Krstic A., Drakulic D., Jovanovic I., Parezanovic V., Stevanovic M. 4q34.1-q35.2 deletion in a boy with phenotype resembling 22q11.2 deletion syndrome. 2011. Eur J Pediatr, 170:1465-70. http://www.ncbi.nlm.nih.gov/pubmed/21833498
  1. Popovic J., Klajn A., Petrovic I., Stevanovic M.Tissue-specific Forkhead protein FOXA2 up-regulates SOX14 gene expression. 2010. Biochim Biophys Acta: Gene Regulatory Mechanisms, 1799: 411–418. http://www.ncbi.nlm.nih.gov/pubmed/20074681
  1. Petrovic I., Kovacevic-Grujicic N., Stevanovic M. Early growth response protein 1 acts as an activator of SOX18 promoter. 2010. Exp Mol Med., 42:132-42. http://www.ncbi.nlm.nih.gov/pubmed/20054233
  1. Mojsin M., Stevanovic M. PBX1 and MEIS1 up-regulate SOX3 gene expression by direct interaction with a consensus binding site within the basal promoter region. 2010. Biochem J, 425:107-16. http://www.ncbi.nlm.nih.gov/pubmed/19799567
  1. Nikčević G., Savić T., Kovačević-Grujičić N., Stevanović M. Up-regulation of the SOX3gene expression by retinoic acid: characterization of the novel promoter-response element and the retinoid receptors involved. 2008. J Neurochem, 107: 1206-15. http://www.ncbi.nlm.nih.gov/pubmed/18786169
  1. Kovačević-Grujičić N., Mojsin M., Krstić A., Stevanović M. Functional characterization of the human SOX3 promoter: Identification of transcription factors implicated in basal promoter activity. 2005. Gene, 344: 287-97. http://www.ncbi.nlm.nih.gov/pubmed/15656994
  1. Stevanovic M. Modulation of SOX2 and SOX3 gene expression during differentiation of human neuronal precursor cell line NTERA2. 2003. Mol Biol Rep, 30: 127-132. http://www.ncbi.nlm.nih.gov/pubmed/12841584
  1. Colignon J., Sockanathan S., Hacker A., Cohen-Tannoudji M., Norris D., Ratan S., Stevanović M., Goodfellow P. N., Lovell-Badge R. A comparison of the properties of           Sox-3 with Sry and two related genes, Sox-1 and Sox-2. 1996. Development, 122: 509-520. http://www.ncbi.nlm.nih.gov/pubmed/8625802
  1. Stevanović M., Zuffardi O., Collignone J., Lovell-Badge R., and Goodfellow P. The cDNA sequence and chromosomal location of the human SOX2 gene. 1994. Mamm Genome, 5: 640-642. http://www.ncbi.nlm.nih.gov/pubmed/7849401
  1. Foster J.W., Dominguez-Steglich M.A., Guioli S., Kwok C., Weller P.A., Stevanović M., Weissenbach J., Mansour S., Young I.D., Goodfellow P.N., Brook J.D., Schafer A.J. Campomelic dysplasia and autosomal sex reversal caused by mutation in an Sry-related gene. 1994. Nature, 372: 525-530. http://www.ncbi.nlm.nih.gov/pubmed/7990924
  1. Stevanović M., Lovell-Badge R., Collignone J., Goodfellow, P. SOX3 is an X-linked        gene related to SRY. 1993. Hum Mol Genet, 2: 2013-2018. http://www.ncbi.nlm.nih.gov/pubmed/8111369
  1. Farr C.J., Stevanović M., Thomson E.J., Goodfellow P., Cooke, H.J. Telomere-associated chromosome fragmentation: applications in genome manipulation and analysis. 1992. Nat Genet, 2: 275-282. http://www.ncbi.nlm.nih.gov/pubmed/1303279
  1. Stevanović M., Paunesku T., Radosavljević D., Drmanac R., Crkvenjakov R. Variant chromosomal arrangement of adult β-globin genes in rat. 1989. Gene, 79: 139-150. http://www.ncbi.nlm.nih.gov/pubmed/2777087