RESEARCH
EDUCATION
SERVICES
INNOVATION

News

  • Call opened for ICGEB meetings and courses in 2019 +

    ICGEB has announced the call for proposals of meetings and
    Read More
  • Call for the CRP - ICGEB Research Grants 2018 +

    International Centre for Genetic Engineering and Biotechnology (ICGEB) has announced
    Read More
  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 8
  • 9
  • 10
  • 11
  • 12
  • 13
  • 14
  • 15
  • 16
  • 17
  • 18
  • 19
  • 20
  • 21
  • 22
  • 23
  • 24
  • 25
  • 26
  • 27
  • 28
  • 29
  • 30
  • 31
  • 32
  • 33
  • 34
  • 35
  • 36
  • 37
  • 38
  • 39
  • 40
  • 41
  • 42

Proba

In the scope of its activities IMGGE offers molecular genetic testing as a service.

Services are performed by the Sector for Laboratory Investigations, which was formed on 24th January 2011 by the Institute’s Governing Board.

Sector for Laboratory Investigations performs genetic testing on the following samples:

  • plant material (seed, food and feed)
  • human biological samples (blood, bone marrow, buccal swab, amniotic fluid, chorionic villi, DNA samples)
  • cytogenetic slides

HUMAN GENETICS

DNA extraction with verification

40 eur
RNA extraction with verification 40 eur

 

INHERITED RARE DISEASES

Clinical exome sequencing

Clinical exome sequencing

 

 

Alpha-1 Antitrypsin Deficiency

Detection of S and Z mutations in А1АТ*

 

 

Cystic fibrosis

Detection of F508del CFTR mutation* 100 eur
Detection of the 7 most common CFTR mutations* 210 eur
Prenatal detection of cystic fibrosis* 170 eur
Screening of the CFTR gene 2* (exons: 2,6b,8,14b,16,17a,18, 22,23,24) 490 eur
Screening of the CFTR gene 1* (exons: 1,3,4,5,6a,7,9,10, 11,12,13,14a,15,17b,19,20,21) 810 eur

 

Phenylketonuria

Detection of mutations in the PAH gene* 380 eur

 

Congenital adrenal hyperplasia

Detection of mutations, conversions and large deletions of the CYP21A2 gene  
Detection of mutations in the CYP21A2 gene - in the family member  

 

Thalassemia

Detection of mutations in β- globin genes* 180 eur
Detection of mutations in α- globin genes* 140 eur
Detection of mutations in α- and β- globin genes in the family member* 75 eur
Prenatal detection of thalassemia syndromes* 75 eur

 

Gilbert's syndrome

Analysis of variants of UGT1A1*28significant as a diagnostic marker in
Gilbert 's syndrome and pharmacogenetic marker during irinotecan therapy
 

MALE INFERTILITY

Detection of the presence of structural and numerical aberrations of sex chromosomes in infertile men
(price per sample for individual analysis)

 

The detection of the presence of structural and numerical aberrations of sex chromosomes in infertile men
(price per sample when analyzed simultaneously 10 or more samples)

 

Detection of numerical aberrations of chromosomes X, Y, 13, 18 and 21 byFISH

 

Detection ofSRY gene on periferal blood samples

46 eur

Detection of SRYgene on amniotic fluid samples

70 eur

Detection of SRY gene on gonadal tissue samples

70 eur

Detection of DNA fragmentation in the sperm samples

 

Detection of DNA fragmentation in the frozen sperm samples

 

THROMBOPHILIA

Thrombofilic set
(FV Leiden, FII G20210A, MTHFR C677T i PAI-1 4G/5G)                                                      

 
Thrombofilic set – 1 mutation*       65 eur
Thrombofilic set – 2 mutations* 90 eur
Thrombofilic set – 3 mutations* 160 eur
Thrombofilic set – 4 mutations* 175 eur

PHARMACOGENETICS

Detection of mutations in TPMT gene*

135 eur

Analysis of polymorphism (VKORC1c.-1639G>A) affecting individual response
to anticoagulant therapy*

63 eur

Analysis of polymorphisms (CYP2C9*2, and CYP2C9*3) affecting individual response
to anticoagulant therapy*

92 eur

Analysis of polymorphisms (VKORC1c.-1639G>A, CYP2C9*2, and CYP2C9*3) affecting individual response
to anticoagulant therapy*

164 eur

Analysis of variants of UGT1А1*28 significant as a diagnostic marker in
Gilbert 's syndrome and pharmacogenetic marker during irinotecan therapy

 

Detection of polymorphism in CYP2D6 gene

 

Detection of polymorphism in CYP2C9 gene

 

Detection of polymorphism in CYP2C19 gene

 

Detection of polymorphism in NАТ2 gene

 

Detection of polymorphism in CYP1А2 gene

 

Detection of polymorphism in CYP3А4 gene

 

Detection of polymorphism in
CYP2D6, CYP2C9, CYP2C19, NAT2, CYP1A2, CYP3A4 gene

 
 

VETERINARY

Analysis of bovine k – CASEIN gene variants

38 eur

Analysis of bovine ß – LACTOGLOBULIN gene variants

38 eur

Molecular diagnostics of BLAD (Bovine Leukocyte Adhesion Deficiency)

46 eur

Molecular diagnostics of Porcine Stress Syndrome (PSS)

 

MICROBIOLOGY

Molecular diagnostics of microorganisms

 

Purchase of the reference strain

 

"DNK fingerprinting" analysis microorganisms
(with existing primers and reference strains)

 

Determination of an unknown microorganism 16s rDNA sequencing the region

 

Starter cultures for production of fermented milk products

 

PFGE genotyping
(Price is for genotyping of 1 to 20 microorganizms)

 

Determining the total number of viable bacteria / yeast sample
(Price is for cultivation of one bacteria in the standard medium it would be changed according to the number,
type of microorganism and the needed medium)

 

DNA isolation, purification of DNA, PCR and DNA sequencing analysis, data processing
(Price is for determination of one bacteria in the standard medium it would be changed according to the number,
type of microorganism and the needed medium)

 

Isolation , purification and PCR with suitable primers
(Price is for isolation of 1 to 10 microorganizms)

 

GENERAL

PCR analysis with verification
(primers are not included)

 

Designing primers
(per primer)

 

Isolation of total protein

 

SDS-PAGE

 

Education

 

Remark:*Services accredited at the Accreditation Body of Serbia in accordance with the requirements SRPS ISO/IEC 17025:2006 (accreditation number 01-363).

Circadian regulation of phototropism in Arabidopsis and potato

Principal Investigator:
Dr Dragan Vinterhalter, Institute for Biological Research "Sinisa Stankovic"

            The ability to sense and appropriately respond to changes in their environment contributes to the survival of living organisms. Interestingly, in a few instances this capacity was shown to be modulated or gated by the circadian clock. Phototropism (PT) is classic photobiological response that has attracted the attention of scientists for centuries. It enables plants growing in low light environments to tune the position of their photosynthetic organs and thus optimize photosynthesis. Although most initial studies were conducted in etiolated grass (monocotyledonous) seedlings, most recent work was performed in Arabidopsis thaliana. This lead to the identification of the phototropic photoreceptors (phot1 and phot2 in Arabidopsis), a photoreceptor family that is present in all plants. Moreover, a number of important steps leading from light perception to initiation of asymmetric growth in the photostimulated organ have been identified. A key event leading to tropic growth is the establishment of an auxin gradient. In addition, other hormones may also contribute to this response. Despite its clear advantages for conducting molecular genetic research Arabidopsis is not always the most convenient model. Moreover, recent studies have shown that studying phototropism in multiple species may help in defining conserved elements of the pathway. Recent work from the Vinterhalter group indicates that in potato plantlets phototropic responsiveness is gated by the circadian clock. Circadian modulation of phototropism in potato may be related to leaf movements tracking the sun during the day, while at night the leaves return to the “morning position” anticipating dawn. Based on this exciting novel finding we propose the following research aims: 1. Is circadian-regulated PT in potato correlated with rhythmic expression of the photoreceptor? 2. Is circadian-regulated PT in potato correlated with rhythmic levels of growth hormones? 3. Is circadian-regulated PT in potato modulated by sugar availability? 4. Do phytochromes modulate PT in potato? 5. Developmental modulation of the phototropic response in Arabidopsis. 6. Is PT in Arabidopsis regulated by the circadian clock? With this proposal we want to address the molecular mechanisms underlying clock controlled growth towards the light by using simple and complementary models (potato and Arabidopsis seedlings). Moreover the collaboration between the Vinterhalter and Fankhauser lab will contribute to an important technology transfer to the Department of Plant Physiology in Belgrade (Serbia) that will result both in upgraded facilities and training of the next generation of plant physiologists.